Composite

Part:BBa_K3628024:Design

Designed by: Tianzuo Zeng   Group: iGEM20_SMS_Shenzhen   (2020-10-27)


J23106-RBS-T7 RNA polymerase N 1~564-Vvd-RBS-Vvd-T7 RNA polymerase C 565~883


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1953
    Illegal AgeI site found at 2181
    Illegal AgeI site found at 2498
    Illegal AgeI site found at 2726
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We construct this composite sequence to regulate the expression of sequences after T7 promoter.


Source

We get this by assembling several parts.

References